The C-terminally truncated β-galactosidase enzyme from Bacillus circulans ATCC 31382 is a member of Glycoside Hydrolase family 2. This enzyme, also known as BgaD-D, is the shortest isoform of full-length protein BgaD. The microbial enzyme is able to synthesize prebiotic galactooligosaccharides (GOS) and Oligos from lactulose (OsLu). These products are widely used as biocatalyst in the food industry, because of their healthy effect on humans. GOS mixtures mimic the prebiotic effects of the human milk oligosaccharides, and therefore are added to infant formula.
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*Activity was measured by following the release of glucose from lactose. One unit of enzyme activity is defined as the release of 1 μmol of substrate per minute.