The C-terminally truncated β-galactosidase enzyme from Bacillus circulans ATCC 31382 is a member of Glycoside Hydrolase family 2. This enzyme, also known as BgaD-D, is the shortest isoform of full-length protein BgaD. The microbial enzyme is able to synthesize prebiotic galactooligosaccharides (GOS) and Oligos from lactulose (OsLu). These products are widely used as a biocatalyst in the food industry, because of their healthy effect on humans. GOS mixtures mimic the prebiotic effects of the human milk oligosaccharides, and therefore are added to infant formula.
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*Activity was measured by following the release of glucose from lactose. One unit of enzyme activity is defined as the release of 1 μmol of substrate per minute.