The C-terminally truncated β-galactosidase enzyme from Bacillus circulans ATCC 31382 mutant R484S is a member of Glycoside Hydrolase family 2. The microbial enzyme is able to synthesize prebiotic galactooligosaccharides (GOS) and Oligos from lactulose (OsLu). These products are widely used as biocatalyst in the food industry, because of their healthy effect on humans. GOS mixtures mimic the prebiotic effects of the human milk oligosaccharides, and therefore are added to infant formula.
The mutant enzyme displays altered enzyme specificity, leading to new types of GOS products. The GOS mixture produced by the mutant enzyme introduces 14 new structures that were not present in the wild type, 10 of these were never shown before.
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*Activity was measured by following the release of glucose from lactose. One unit of enzyme activity is defined as the release of 1 μmol of substrate per minute.