Glucansucrases

  • The N-terminally truncated glucansucrase Gtf180 of Lactobacillus reuteri, belongs to glycosyl hydrolase family GH70. The enzyme synthesizes branched glucans containing 69 % α(1→6)-, and 31 % α(1→3)-glycosidic linkages (dextran) using sucrose as a substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri strain 180

    Sucrose

    EC 2.4.1.5

    GH70

    4.5

    50

    Solid lyophilized powder

    117

    4

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  • The N- terminally truncated glucansucrase GtfA of Lactobacillus reuteri strain 121, belongs to glycosyl hydrolase family GH70. The enzyme synthesizes branched glucans with 57 % α(1→4), and 43 % α(1→6)-glycosidic linkages (reuteran) using sucrose as a substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri strain 121

    Sucrose

    2.4.1.-

    GH70

    4.7

    50

    Solid lyophilized powder

    118

    4

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  • The N- and V-terminally truncated glucansucrase Gtf180 of Lactobacillus reuteri 180, belongs to the glycosyl hydrolase family GH70. Compared to Gtf180-ΔN, Gtf180-ΔN-ΔV is impaired in polysaccharide synthesis. Instead this enzyme produces a large number of oligosaccharides. The enzyme forms α(1→6)- and α(1→3)-glycosidic linkages using sucrose as a substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
    Select options

    Lactobacillus reuteri strain 180

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    50

    Solid lyophilized powder

    95

    4

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  • The N-terminally truncated enzyme of Lactobacillus reuteri strain ATCC 55730 (LB BIO) is a hydrolytic reuteransucrase producing a soluble glucan, in which the majority ~79 % of the linkages are of the α(1→4) glucosidic type. This reuteran also contains 21 % α(1→6)- linked glucosyl units, and 4,6-disubstituted α-glucosyl units at the branching points.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of  fructose per minute.
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    Lactobacillus reuteri strain ATCC 55730

    Sucrose

    2.4.1-

    GH70

    5.0

    35

    Solid lyophilized powder

    117

    Room temperature

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  • The N-terminally truncated glucansucrase GtfKg15 of Lactobacillus sakei, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with 90 % α(1→6), and 10 % α(1→3) linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
    Select options

    Lactobacillus sakei strain Kg15

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    133

    4

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  • The N- terminally truncated glucansucrase GtfKg3 of Lactobacillus fermentum, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with 90 % α(1→6) and 10 % α(1→3) linkages using sucrose as a substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus fermentum strain Kg3

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    131

    4

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  • The N-terminally truncated glucansucrase Gtf33 of Lactobacillus parabuchneri 33, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with 81 % α(1→6) and 19 % α(1→3) linkages using sucrose as a substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
     
    Select options

    Lactobacillus parabuchneri strain 33

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    142

    4

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  • The N-terminally truncated glucansucrase GtfML1 of Lactobacillus reuteri strain ML1, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes a highly branched mutan-like polymer containing 65 % α(1→3), and 35 % α(1→6) linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
    Select options

    Lactobacillus reuteri strain ML1

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    116

    4

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  • The N-terminally truncated glucansucrase Gtf180 of Lactobacillus reuteri L940W mutant, belongs to glycosyl hydrolase family GH70.  The wild type enzyme synthesizes dextran-like α-glucans containing α(1→6) and α(1→3) glycosidic linkages in presence of sucrose. Instead, the GTF180-ΔN mutant L940W is unable to form (α1→3) linkages in its products. The enzyme synthesizes mainly linear isomalto-oligosaccharides (IMOs) and a minor amount of α-glucans (up to 6.3MDa) using sucrose as a substrate. The products contain α(1→6) glycosidic linkages only. The product size is relatively small compared with the wild type enzyme. Enzyme mutations of Leu940 in domain B significantly changed the linkage specificity, reaction specificity, and activity.

    This product is sold for research use only.
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    Lactobacillus reuteri strain 180

    Sucrose

    EC 2.4.1.5

    GH70

    4.5

    50

    Solid lyophilized powder

    117

    4

    Ask a question Details