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The glucansucrase of Lactobacillus reuteri 180 catalyzes the synthesis of a diversity of α-glucans from sucrose. An enzyme mutation of Leu940 in domain B significantly changed the linkage and reaction specificity. Compared with the GTF180-ΔN wild type, the GTF180-ΔN L940E mutant has an increased percentage of α(1→6) linkages and a decreased percentage of α(1→3) linkages in the product. Furthermore, the mutant enzyme has an increased polysaccharide synthesis.
This product is sold for research use only.
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The glucansucrase of Lactobacillus reuteri 180 catalyzes the synthesis of a diversity of α-glucans from sucrose. An enzyme mutation of Leu940 in domain B significantly changed the linkage and reaction specificity. Compared with the GTF180-ΔN wild type, the GTF180-ΔN L940F mutant has an increased percentage of α(1→6) linkages and a decreased percentage of α(1→3) linkages in the product. Furthermore, the mutant enzyme has an increased polysaccharide synthesis.
This product is sold for research use only.
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The mutan like polymer GtfML1 is formed using sucrose by the glucanotransferase GtfML1 of Lactobacillus reuteri strain ML1. The highly branched compound contains mainly α(1→3) and a minor amount of α(1→6) linkages. Glucans produced by glucanotransferase enzymes are potentially prebiotic, stimulating the gastrointestinal tract.
This product is sold for research use only.
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The polysaccharide GtfA reuteran is a unique, soluble glucan produced from sucrose by the glucansucrase GtfA-ΔN of Lactobacillus reuteri strain 121. The branched structure contains mainly α(1→4) and α(1→6) glucosidic linkages, with no repeating units present. The production of glucans using lactic acid bacteria is Generally Recognized as Safe (GRAS), and the compounds have potential prebiotic properties.
This product is sold for research use only.
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The polysaccharide GtfO-∆N reuteran is a soluble glucan produced from sucrose by the glucansucrase GtfO of Lactobacillus reuteri ATCC 55730 (LB BIO). The branched structure contains mainly 79 % α(1→4) and a minor amount of 21 % α(1→6) glycosidic linkages, without repeating units present. The production of glucans using lactic acid bacteria is Generally Recognized as Safe (GRAS), and the compounds have potential prebiotic properties.
This product is sold for research use only.
