The N-terminally truncated glucansucrase GtfML1 of Lactobacillus reuteri strain ML1, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes a highly branched mutan-like polymer containing 65 % α(1→3), and 35 % α(1→6) linkages using sucrose as substrate.

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*Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.