Slowly digestible carbohydrates & fibers

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  • The polysaccharide GtfA reuteran is a unique, soluble glucan produced from sucrose by the glucansucrase GtfA-ΔN of Lactobacillus reuteri strain 121. The branched structure contains mainly α(1→4) and α(1→6) glucosidic linkages, with no repeating units present. The production of glucans using lactic acid bacteria is Generally Recognized as Safe (GRAS), and the compounds have potential prebiotic properties.

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    Lactobacillus reuteri strain 121

    Sucrose

    45

    α(1→4) and α(1→6)

    Yes

    58 % (α1→4), 42 % (α1→6)

    11 % Glcp-(1→, 46 % →4)-Glcp-(1→, 26 % →6)-Glcp-(1→, 17 % →4,6)-Glcp-(1→

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  • The polysaccharide GtfO-∆N reuteran is a soluble glucan produced from sucrose by the glucansucrase GtfO of Lactobacillus reuteri ATCC 55730 (LB BIO). The branched structure contains mainly 79 % α(1→4) and a minor amount of 21 % α(1→6) glycosidic linkages, without repeating units present. The production of glucans using lactic acid bacteria is Generally Recognized as Safe (GRAS), and the compounds have potential prebiotic properties.

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    Lactobacillus reuteri strain ATCC 55730

    Sucrose

    42

    α(1→4) and α(1→6)

    Yes

    79 % (α1→4), 21 % (α1→6)

    11 % Glcp-(1→, 69 % →4)-Glcp-(1→, 9 % →6)-Glcp-(1→, 11 % →4,6)-Glcp-(1→

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  • The exopolysaccharide Gtf180 dextran is produced by the glucansucrase of Lactobaccillus reuteri 180 using sucrose as substrate. The heavily branched α-D-glucan has a heterogeneous structure containing α(1→6) and α(1→3) glucosidic linkages without repeating units present. The terminal positions only carries -α-D-Glcp-(1→6) units, whether all the -α-D-Glcp-α(1→3)- units are 6-substituted. The polysaccharide is constructed out of different isomalto-oligosaccharides (IMO) structures, connected by single α(1→3) bridges in linear and branched orientations.

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    Lactobacillus reuteri strain 180

    Sucrose

    22.6

    α(1→6) and α(1→3)

    Yes

    69 % (α1→6), 31 % (α1→3)

    11 % Glcp-(1→, 23 % →3)-Glcp-(1→, 54 % →6)-Glcp-(1→, 13 % →3,6)-Glcp-(1→

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  • The glucansucrase of Lactobacillus reuteri 180 catalyzes the synthesis of a diversity of α-glucans from sucrose. An enzyme mutation of Leu940 in domain B significantly changed the linkage and reaction specificity. Compared with the GTF180-ΔN wild type, the GTF180-ΔN L940E mutant has an increased percentage of α(1→6) linkages and a decreased percentage of α(1→3) linkages in the product. Furthermore, the mutant enzyme has an increased polysaccharide synthesis.

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    Lactobacillus reuteri strain 180

    Sucrose

    18.7

    α(1→6) and α(1→3)

    Yes

    73 % (α1→6), 27 % (α1→3)

    Data is not available

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  • The glucansucrase of Lactobacillus reuteri 180 catalyzes the synthesis of a diversity of α-glucans from sucrose. An enzyme mutation of Leu940 in domain B significantly changed the linkage and reaction specificity. Compared with the GTF180-ΔN wild type, the GTF180-ΔN L940F mutant has an increased percentage of α(1→6) linkages and a decreased percentage of α(1→3) linkages in the product. Furthermore, the mutant enzyme has an increased polysaccharide synthesis.

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    Lactobacillus reuteri strain 180

    Sucrose

    19.5

    α(1→6) and α(1→3)

    Yes

    93 % (α1→6), 7 % (α1→3)

    Data is not available

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  • The dextran like polymer GtfKg15 is formed using sucrose by the glucanotransferase GtfKg15 of Lactobacillus sakei strain Kg15. The branched compound contains mainly α(1→6) and a minor amount of α(1→3) linkages. Glucans produced by glucanotransferase enzymes are potentially prebiotic, stimulating the gastrointestinal tract.

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    Lactobacillus sakei strain Kg15

    Sucrose

    27

    α(1→6) and α(1→3)

    Yes

    90 % α(1→6), 10 % α(1→3)

    4 % Glcp-(1→, 1 % →3)-Glcp-(1→, 86 % →6)-Glcp-(1→, 9 % →3,6)-Glcp-(1→

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  • The dextran like polymer GtfKg3 is formed using sucrose by the glucanotransferase GtfKg3 of Lactobacillus fermentum strain Kg3. The branched compound contains mainly α(1→6) and a minor amount of α(1→3) linkages. Glucans produced by glucanotransferase enzymes are potentially prebiotic, stimulating the gastrointestinal tract.

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    Lactobacillus fermentum strain Kg3

    Sucrose

    24

    α(1→6) and α(1→3)

    Yes

    92 % α(1→6), 8 % α(1→3)

    3 % Glcp-(1→, 1 % →3)-Glcp-(1→, 89 % →6)-Glcp-(1→, 7 % →3,6)-Glcp-(1→

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  • The dextran like polymer Gtf33 is formed using sucrose by the glucanotransferase Gtf33 of Lactobacillus parabuchneri strain 33. The branched compound contains a mainly α(1→6) and a minor amount of α(1→3) linkages. Glucans produced by glucanotransferase enzymes are potentially prebiotic, stimulating the gastrointestinal tract.

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    Lactobacillus parabuchneri strain 33

    Maltose

    0.2

    α(1→6) and α(1→3)

    Yes

    81 % α(1→6), 19 % α(1→3)

    6 % Glcp-(1→, 9 % →3)-Glcp-(1→, 75 % →6)-Glcp-(1→, 9 % →3,6)-Glcp-(1→

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  • The mutan like polymer GtfML1 is formed using sucrose by the glucanotransferase GtfML1 of Lactobacillus reuteri strain ML1. The highly branched compound contains mainly α(1→6) and a minor amount of α(1→3) linkages. Glucans produced by glucanotransferase enzymes are potentially prebiotic, stimulating the gastrointestinal tract.

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    Lactobacillus reuteri strain ML1

    Sucrose

    8

    α(1→6) and α(1→3)

    Yes

    65 % α(1→6), 35 % α(1→3)

    18 % Glcp-(1→, 47 % →3)-Glcp-(1→, 10 % →6)-Glcp-(1→, 26 % →3,6)-Glcp-(1→

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