The Gtf180-ΔN-Q1140E mutant of the generally recognized as safe (GRAS) enzyme Lactobacillus reuteri is used to glycosylate the steviol glycoside RebaudiosideA (RebA), wherby sucrose is used as donor substrate. The enzyme glycosylates exclusively the Glc(β1→C-19 residue of the RebA, with the initial formation of an α(1→6) linkage. The core structure is extended with an extra glucose forming RebA-G1 (70 percent), and is multiglycosylated forming RebA-G≥2 (30 percent). The glycosylated RebA product shows a significant reduction in bitterness, resulting in a superior taste profile compared to RebA.
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