Please check-out our open-access paper Valk-Weeber, R. L. et al. Quantitative analysis of bovine whey glycoproteins using the overall N-linked whey glycoprofile. International Dairy Journal, Volume 110, p. 104814. DOI 10.1016/j.idairyj.2020.104814 in collaboration with Friesland Campina (Amersfoort).
Bovine whey is an important ingredient in human nutrition and contains many biofunctional, glycosylated proteins. Knowledge on the glycoprotein composition of whey and whey products is valuable for the dairy industry. This paper describes a method for the characterisation of whey, or whey powders, by N-linked glycoprofile analysis. Application of the method for analysis of whey protein products showed clear differences in glycoprotein composition between concentrate, isolate, and demineralised whey powders. The quantitative potential was explored by screening 100 pooled farm milk samples. IgG and lactoferrin protein concentrations determined by N-glycoprofile analysis matched well with ELISA results. The protein concentration of GlyCAM-1 was determined to be ≥1 mg mL−1. The approaches presented in this work allow simultaneous concentration estimation of the three major whey glycoproteins lactoferrin, IgG, and GlyCAM-1 on the basis of their N-linked glycoprofiles, also in highly processed samples where conventional methods of detection (ELISA) are less suitable.