Get in-depth knowledge of your carbohydrate products

Carbohydrates are increasingly important food ingredients, serving many purposes such as texturizing and energy release. In other industries, a wide variety of functionalities make carbohydrates suitable for e.g. coatings, drug delivery, and bioplastic applications. All carbohydrates share the property that their monosaccharide constituents are connected through glycosidic linkages. Both the monosaccharide constituents and the type of linkages account for the 3D-structure and hence the functionality of the carbohydrate.

CarbExplore has developed a broad range of carbohydrate fingerprinting techniques. We screen for oligosaccharides, polysaccharides, glycoproteins, glucosamines, glucuronic acids, sialic acids and other carbohydrate derivatives. Our scientifically established methods are available to support your quality control of raw materials and products. For novel substances and impurities, we offer extensive carbohydrate structure elucidation.

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Example: Maltose

Maltose consists of 2 glucose units linked through an α(1→4) glycosidic bond.

After derivatization, the maltose is analyzed with a GC-MS, where the produced A and B fractions leave a characteristic fingerprint.

Workflow:


You provide:

✔ At least 250 µg of carbohydrates (dried, desalted, free of detergent)

✔ ! For glycoproteins, at least 1 mg


We execute the following techniques for the carbohydrate structure analyses:

✔ Partially methylated alditol acetate (PMAA) derivatization for linkage analysis

✔ Trimethylsilyl (TMS) derivatization for monosaccharide composition analysis

✔ Alditol acetate (AA) derivatization for monosaccharide composition analysis

✔ GC-MS analysis

 

We also use the following analytical techniques:

  • NMR spectroscopy
  • HPLC-UV
  • HPLC-FLD
  • HPAEC-PAD