The C-terminally truncated β-galactosidase enzyme of Bacillus circulans ATCC 31382 derived oligosaccharides from lactulose (OsLu). OsLu may act as prebiotic compounds and are potentially resistant to gut digestion.

Five oligosaccharide structures were identified using HPAEC-PAD chromatography,  Maldi-ToF-MS, and NMR analysis. The products of the enzyme have a preference for introducing β(1→4) linkages. The trisaccharide β-D-Galp-(1 → 4)-β-D-Galp-(1 → 4)-D-Fru is the most abundant compound in the mixture.

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