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Oligos (GOS) from lactose, synthesized by BgaD-D of B. circulans ATCC 31382

250.00

Galacto-oligosaccharides (GOS) are widely used as a biocatalyst in the food industry. GOS mixtures mimic the prebiotic effects of the human milk oligosaccharides, by stimulating the number of bifidobacteria and their metabolic activity in the gut, and therefore are added to infant formula.

The Galacto-oligosaccharides (GOS) product synthesized by the enzyme BgaD-D presents a carbohydrate mixture containing a majority of β(1→4) linked galactose on the reducing glucose residue, and a trace amount of β(1→2), β(1→3), β(1→6) linkages. A total of 15 compounds have been identified in the wild type GOS compounds. The products contain both linear and branched structures. Some structures have been further elongated with β(1→4)-linked galactose residues.

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SKU: CE-GOS-1 Categories: ,

Bacillus circulans ATCC 31382

Lactose

D-Galp (galactose) (1), D-Glcp (glucose) (2), β-D-Galp-(1→6)-D-Glcp (4), β-D-Galp-(1→4)-D-Glcp (lactose) (5), β-D-Galp-(1→4)- β-D-Glcp-(1→6)-D-Galp (6a), β-D-Galp-(1→4)-D-Galp (7), β-D-Galp-(1→2)-D-Glcp (8a), β-D-Galp-(1→3)-D-Glcp (8b), β-D-Galp-(1→4)- β-D-Glcp-(1→2)-D-Galp (9), β-D-Galp-(1→6)- β-D-Glcp-(1→2)-D-Galp (10a), β-D-Galp-(1→6)- β-D-Glcp-(1→3)-D-Galp (10b), β-D-Galp-(1→4)-β-D-Galp-(1→4)-D-Glcp (11), β-D-Galp-(1→3)-β-D-Galp-(1→4)-D-Glc (12), β-D-Galp-(1→4)-β-D-Galp-(1→2)-D-Glcp (13a), β-D-Galp-(1→4)-β-D-Galp-(1→3)-D-Glcp (13b), β-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Galp-(1→4)-D-Glcp (17), β-D-Galp(1→4)-β-D-Galp-(1→4)-β-D-Galp-(1→2)-D-Glcp (18a), β-D-Galp(1→4)-β-D-Galp-(1→3)-β-D-Galp-(1→3)-D-Glcp (18b)

2-5

ß(1→4), ß(1→2), ß(1→3), and ß(1→6)

Yes

Lyophilized powder

C12H22O11 (4-5, 7-8,) C18H32O16 (6a, 9-13) C24H42O21 (17)

342.3 (4-5, 7-8,) 504.3 (6a, 9-13) 666.3 (17)

water

4

Description

Galacto-oligosaccharides (GOS) are widely used as a biocatalyst in the food industry. GOS mixtures mimic the prebiotic effects of the human milk oligosaccharides, by stimulating the number of bifidobacteria and their metabolic activity in the gut, and therefore are added to infant formula.

Compared with commercial available β-galactosidase enzymes, the BgaD-D enzyme of Bacillus circulans ATCC 31382 generates a high yield of GOS showing a complex formation pattern. The GOS product synthesized by the wild type BgaD-D presents a carbohydrate mixture showing a majority of β(1→4) linked galactose on the reducing glucose residue, and a trace amount of β(1→2), β(1→3), β(1→6) linkages. A total of 15 compounds have been identified in the wild type GOS compounds. The products contain both linear and branched structures. Some structures have been further elongated with β(1→4)-linked galactose residues.

Human newborns are capable to produce β-galactosidase enzymes to digest lactose. Nevertheless, the majority of adults have lost the ability to produce these enzymes. This can end up in lactose intolerance, making them incapable to digest dairy products. The lactose could be removed from the milk to solve this problem, and the residual product could be converted to high valuable lactose derivates like GOS.

Additional information

Volume

100 mg

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  • Select options

    Bacillus circulans ATCC 31382

    Lactose and lactulose

    3.2.1.23

    GH2

    6.0

    60

    Lyophilized powder

    189

    4

    Ask a question Details
  • Select options

    Bacillus circulans ATCC 31382

    Lactose

    D-Galp (galactose) (1), D-Glcp (glucose) (2), β-D-Galp-(1→6)-D-Glcp (4), β-D-Galp-(1→4)-D-Glcp (β-lactose) (5), β-D-Glcp-(1→3)-D-Glcp (38), β-D-Galp-(1→4)- β-D-Glcp-(1→6)-D-Galp (6a), β-D-Galp-(1→4)-D-Galp (7), β-D-Galp-(1→2)-D-Glcp (8a), β-D-Galp-(1→3)-D-Glcp (8b), β-D-Galp-(1→4)- β-D-Glcp-(1→2)-D-Galp (9), β-D-Galp-(1→6)- β-D-Glcp-(1→2)-D-Galp (10a), β-D-Galp-(1→6)- β-D-Glcp-(1→3)-D-Galp (10b), β-D-Galp-(1→6)-β-D-Glcp-(1→4)-β-D-Glcp-(1→6)-D-Galp (39a), β-D-Galp-(1→3,4)-β-D-Glcp-(1→4)-D-Galp (39b), β-D-Galp-(1→4)-β-D-Galp-(1→4)-D-Glcp (11), β-D-Galp-(1→3)-β-D-Galp-(1→4)-D-Glcp (12), β-D-Galp-(1→3)-β-D-Glcp-(1→2)-β-D-Glcp-(1→6)-D-Galp (40), β-D-Galp-(1→4)-β-D-Galp-(1→2)-D-Glcp (13a), β-D-Galp-(1→4)-β-D-Galp-(1→3)-D-Glcp (13b), β-D-Galp-(1→3)-β-D-Galp-(1→3)-D-Glcp (29), β-D-Galp-(1→3)-β-D-Galp-(1→2)-D-Glcp (30), β-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Galp-(1→4)-D-Glcp (17), β-D-Galp-(1→3)-β-D-Galp-(1→4)-β-D-Galp-(1→4)-D-Glcp (41), β-D-Galp(1→3)-β-D-Galp-(1→3)-β-D-Galp-(1→4)-D-Glcp (31), β-D-Galp(1→4)-β-D-Galp-(1→3)-β-D-Galp-(1→4)-D-Glcp (42), β-D-Galp(1→4)-β-D-Galp-(1→4)-β-D-Galp-(1→2)-D-Glcp (18a), β-D-Galp(1→4)-β-D-Galp-(1→4)-β-D-Galp-(1→3)-D-Glcp (18b), β-D-Galp(1→3)-β-D-Galp-(1→3)-β-D-Galp-(1→4)-β-D-Galp-(1→4)-D-Glcp (43), β-D-Galp(1→3)-β-D-Galp-(1→4)-β-D-Galp-(1→4)-β-D-Galp-(1→4)-D-Glcp (44a), β-D-Galp(1→4)-β-D-Galp-(1→3)-β-D-Galp-(1→3)-β-D-Galp-(1→4)-D-Glcp (44b), β-D-Galp(1→4)-β-D-Galp-(1→3)-β-D-Galp-(1→4)-β-D-Galp-(1→4)-D-Glcp (45), β-D-Galp(1→3)-β-D-Galp-(1→4)-β-D-Galp-(1→3)-β-D-Galp-(1→4)-D-Glcp (46)

    2-5

    ß(1→3), ß(1→4), ß(1→6), and ß(1→2)

    Yes

    Lyophilized powder

    C12H22O11 (4-5, 7-8, 38) C18H32O16 (6a, 9-13, 29-30) C24H42O21 (17, 31, 39-42) C24H42O21 (43-46)

    342.3 (4-5, 7-8, 38) 504.3 (6a, 9-13, 29-30) 666.3 (17, 31, 39-42) 828,3 (43-46)

    water

    4

    Ask a question Details
  • Select options

    Bacillus circulans ATCC 31382

    Lactose and lactulose

    3.2.1.23

    GH2

    6.0

    60

    Lyophilized powder

    189

    4

    Ask a question Details