The N- and V-terminally truncated glucansucrase Gtf180 of Lactobacillus reuteri 180, belongs to the glycosyl hydrolase family GH70. Compared to Gtf180-ΔN, Gtf180-ΔN-ΔV is impaired in polysaccharide synthesis. Instead this enzyme produces a large number of oligosaccharides. The enzyme forms α(1→6)- and α(1→3)-glycosidic linkages using sucrose as a substrate.

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*Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.