BgaD-D of Bacillus circulans ATCC 31382

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BgaD-D of Bacillus circulans ATCC 31382

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The C-terminally truncated β-galactosidase enzyme from Bacillus circulans ATCC 31382 is a member of Glycoside Hydrolase family 2. This enzyme, also known as BgaD-D, is the shortest isoform of full-length protein BgaD. The microbial enzyme is able to synthesize prebiotic galactooligosaccharides (GOS) and Oligos from lactulose (OsLu). These products are widely used as a biocatalyst in the food industry, because of their healthy effect on humans. GOS mixtures mimic the prebiotic effects of the human milk oligosaccharides, and therefore are added to infant formula.

This product is sold for research use only.
*Activity was measured by following the release of glucose from lactose. One unit of enzyme activity is defined as the release of 1 μmol of substrate per minute.

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SKU: CE-ENZ-BGAL-1 Categorieën: ,

Bacillus circulans ATCC 31382

Lactose and lactulose

3.2.1.23

Beschrijving

The C-terminally truncated β-galactosidase enzyme from Bacillus circulans ATCC 31382 is a member of Glycoside Hydrolase family 2. This enzyme, also known as BgaD-D, is the shortest isoform of the full-length protein BgaD. The microbial enzyme is able to synthesize prebiotic galactooligosaccharides (GOS) and Oligos from lactulose (OsLu). These products are widely used as biocatalyst in the food industry, because of their healthy effect on humans. GOS mixtures mimic the prebiotic effects of the human milk oligosaccharides, and therefore are added to infant formula.

Compared with commercial available β-galactosidase enzymes, the BgaD-D enzyme of B. circulans generates a high yield of GOS showing a complex formation pattern. The GOS product synthesized by the BgaD-D protein presents a carbohydrate mixture which contains a majority of β(1→4) linked galactose on the reducing glucose residue, and a trace amount of β(1→2), β(1→3), β(1→6) linkages. The products contain both linear and branched structures. Some structures have been further elongated with β(1→4)-linked galactose residues.

Moreover, the BgaD-D enzyme is able to synthesize oligosaccharides out of lactulose (OsLu). Although the yields are rather low compared with GOS production using lactose, the enzyme creates high-value compounds. The products of this β-galactosidase have a preference for introducing β(1→4) and β(1→3) linkages. OsLu may act as prebiotic compounds and are potentially resistant to gut digestion.

Human newborns are capable to produce β-galactosidase enzymes to digest lactose. Nevertheless, the majority of adults have lost the ability to produce these enzymes. This can end up in lactose intolerance, making them incapable to digest dairy products. The lactose could be removed from the milk to solve this problem, and the residual product could be converted to high valuable lactose derivates like GOS.

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1000 units, 300 units

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