The glucansucrase of Lactobacillus reuteri 180 catalysis the synthesis of a diversity of α-glucans from sucrose. An enzyme mutation of Leu940 in domain B significantly changed the linkage and reaction specificity. Compared with GTF180-ΔN, the GTF180-ΔN L940F mutant has an increased percentage (α1→6) linkages, and a decreased percentage of (α1→3) linkages in the product. Furthermore, the enzyme mutant increased polysaccharide synthesis.

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