The Lactobacillus reuteri strain ML1 N-terminally truncated glucansucrase GtfML1, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes a highly branched mutan-like polymer containing α(1→3) (65 %) and α(1→6) (35 %) linkages using sucrose as substrate.

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*Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.