GtfC 4,6-α-glucanotransferase of Exiguobacterium sibiricum 255-15

CarbExplore

Schematic view of the domain order in GH13 a-amylases and GH70 glucansucrases and 4,6-glucanotransferases. GtfC-like enzymes likely represent an evolutionary intermediate between the GH13 -amylases (left) and the GH70 glucansucrases and GtfB-like 4,6-glucanotransferases (right).

GtfC 4,6-α-glucanotransferase of Exiguobacterium sibiricum 255-15

Request quote for price

The GtfC enzyme of Exiguobacterium sibiricum 255-15 is a member of the GH70 family. This glucanotransferase is active on malto-oligosaccharides and starch, synthesizing isomalto/malto-oligosaccharides (IMMOs) containing 52 % α(1→6) and 35 % α(1→4)-glycosidic linkages using amylose V. Biochemically, GtfC enzymes are close related to the GH70 GtfB enzymes. Although its domain organization is more closely related to GH13 enzymes (α-amylases). Making the GtfC 4,6-glucanotransferase an interesting intermediate between α-amylase and glucansucrase enzymes.

This product is sold for research use only.

*Enzyme activity was determined by the iodine-staining assay using amylose as substrate. One unit of activity is defined as the amount of enzyme preparation converting 1 mg of substrate per min.

Volume

Verwijder selectie

Amount

Amount

Product price:
Additional options total:
Order total:

SKU: CE-ENZ-GT-2 Categorieën: Carbohydrate-Active enZYmes, Glucanotransferases

Source

Exiguobacterium sibiricum strain 255-15

Substrate

Malto-oligosaccharides/ Amylose

EC number

2.4.1.-

Genbank

CAZy family

pH optimum

Optimum temperature (°C)

Physical form

Mw enzyme (kDa)

Storage temperature (°C)

Gangoiti, J., Pijning, T. & Dijkhuizen, L., 2016. The Exiguobacterium sibiricum 255-15 GtfC enzyme represents a novel glycoside hydrolase 70 subfamily of 4,6-α-glucanotransferase enzymes. Applied and Environmental Microbiology, 82(2), pp. 756-766. DOI10.1128/AEM.03420-15

Extra informatie

Volume	1000 units, 300 units