GtfB-ΔN 4,3-α-glucanotransferase of Lactobacillus fermentum strain NCC 2970
The N-terminally truncated GtfB enzyme of Lactobacillus fermentum NCC 2970 is a member of the GH70 family. The 4,3-α-glucanotransferase converts amylose and malto-oligosaccharides in an alternating glucan containing 60 % α(1→4) and 40 % α(1→3) glucosidic linkages, and α(1→3,4) branching points.
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*Enzyme activity was determined by the iodine-staining assay using amylose as substrate. One unit of activity is defined as the amount of enzyme preparation converting 1 mg of substrate per min.