Oligos (GOS) from lactose, synthesized by BgaD-D of Bacillus circulans ATCC 31382 mutant R484H
The C-terminally truncated β-galactosidase enzyme of Bacillus circulans ATCC 31382, also known as BgaD-D, is able to synthesize prebiotic galactooligosaccharides (GOS). These products are widely used as a biocatalyst in the food industry, because of their healthy effect on humans. GOS mixtures mimic the prebiotic effects of the human milk oligosaccharides, and therefore are added to infant formula.
The enzyme BgaD-D mutant R484S displays altered enzyme specificity, leading to new types of GOS products. The GOS mixture produced by the mutant enzyme introduces 14 new structures that were not present in the wild type, 10 of these were completely novel structures (39-46). The other four structures were identified in earlier experiments (38, 29-31). In contrast with the wild type, the GOS products synthesized by BgaD-D mutant R484S presents a carbohydrate mixture showing a majority of β(1→3) and β(1→4) linked galactose on the reducing glucose residue. The products contain both linear and branched structures. Some basic structures are further elongated with β(1→3) and β(1→4)-linked galactose residues, resulting in 3,4-disubstituted galactose residues.
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