The N-terminally truncated glucansucrase Gtf33 of Lactobacillus parabuchneri 33, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with 81 % α(1→6) and 19 % α(1→3) linkages using sucrose as a substrate.
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*Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.