The GtfW of Lactobacillus reuteri strain DSM 20016 is a member of the glycoside hydrolase GH70 family. The enzyme is a 4,6-α-glucanotransferase converting pure malto-oligosaccharide substrates (≥ maltose dp 2) into linear α-glucan product mixtures with a glycosidic bonds ratio of 50 % α(1→4) and 50 % α(1→6).

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*Activity was measured by following the release of glucose from amylose. One unit of enzyme activity is defined as the release of 1 μmol of substrate per minute.