The C-terminally truncated inulinosucrase (Inu∆699) of Lactobacillus reuteri strain 121 is an fructosyltransferase belonging to the glycoside hydrolase family 68. This enzyme synthesizes high molecular weight β(2→1)- linked fructan polymers of > 10 MDa, and oligosaccharides (95 % 1-kestose and 5 % nystose) using sucrose as substrate.

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*Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.