The N- terminally truncated glucansucrase GtfA of Lactobacillus reuteri strain 121, belongs to glycosyl hydrolase family GH70. The enzyme synthesizes branched glucans with 57 % α(1→4), and 43 % α(1→6)-glycosidic linkages (reuteran) using sucrose as a substrate.

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*Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.