The Lactobacillus parabuchneri 33 N-terminally truncated glucansucrase Gtf33, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with (1→6) linkages using sucrose as substrate.

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*Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.