The C-terminal truncated levansucrase (Lev∆773) of Lactobacillus reuteri strain 121 belongs to the glycoside hydrolase family 68. This enzyme synthesizes a linear levan polymer containing β(2→6)- linked fructan units, and a low amount (2-3 %) of branching β(1→2) points, using sucrose as substrate.
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*Activity was measured by following the release of glucose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of glucose per minute.