Carbohydrate-Active enZYmes

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  • The GtfW of Lactobacillus reuteri strain DSM 20016 is a member of the glycoside hydrolase GH70 family. The enzyme is a 4,6-α-glucanotransferase converting pure malto-oligosaccharide substrates (≥ maltose dp 2) into linear α-glucan product mixtures with a glycosidic bonds ratio of 50 % α(1→4) and 50 % α(1→6).

    This product is sold for research use only.
    *Activity was measured by following the release of glucose from amylose. One unit of enzyme activity is defined as the release of 1 μmol of glucose per minute.
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    Lactobacillus reuteri strain DSM 20016

    Malto-oligosaccharides

    2.4.1.-

    GH70

    4,5

    40

    Solid lyophilized powder

    154

    4

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  • The GtfML4 of Lactobacillus reuteri strain ML1 is a member of the glycoside hydrolase family GH70. The enzyme is a 4,6-α-glucanotransferase converting pure malto-oligosaccharide substrates (≥ Dp 4) into linear oligosaccharides and polysaccharides. The products formed by maltohexaose and maltheptaose (Dp 6 en 7) conttain ~50 % α(1→4)-, and  ~50 % α(1→6) linkages.

    This product is sold for research use only.
    *Activity was measured by following the release of glucose from amylose. One unit of enzyme activity is defined as the release of 1 μmol of glucose per minute.
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    Lactobacillus reuteri strain ML1

    Malto-oligosaccharides

    2.4.1.-

    GH70

    4,5

    40

    Solid lyophilized powder

    180

    4

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  • The N-terminally truncated GtfB enzyme of Lactobacillus fermentum NCC 2970 is member of the GH70 family. The enzyme acts as 4,3-α-glucanotransferase converting amylose in an alternating α(1→4):α(1→3)-linked α-glucan with a ratio of 60:40 %  containing α(1→3,4) branching points.

    This product is sold for research use only.
    *Activity was measured by following the release of glucose from amylose. One unit of enzyme activity is defined as the release of 1 μmol of glucose per minute.
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    Lactobacillus fermentum strain NCC 2970

    Amylose V

    2.4.1.-

    GH70

    5,5

    50

    Solid lyophilized powder

    110

    4

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  • The GtfD 4,6-α-glucanotransferase enzyme of Paenibacillus beijingensis  is member of the glycoside hydrolase family 70. It produces two reuteran-like polymers distributions were generated from amylose V: a high-molecular mass polymer (HMMP) and a low-molecular mass polymer (LMMP) containing both long linear α(1→4) chains. The HMM polymer with an average lenght of 27 MDa has a linkage ratio α(1→4):α(1→6)= 71:29, and the LMM polymer (19 KDa) has a linkage ratio α(1→4):α(1→6) = 77: 23 %, indicating the HMM has a slighly higher amount of α(1→6) linkages.

    This product is sold for research use only.
    *Activity was measured by following the release of glucose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Azotobacter chroococcum

    n.a.

    2.4.1.-

    GH70

    7,0

    50

    Solid lyophilized powder

    85

    4

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  • The GtfD enzyme of Azotobacter chroococcum is member of the glycoside hydrolase family GH70. The enzyme displays 4,6-α-glucanotransferase activity using malto-oligosaccharides (> DP 4), and starch. However, the enzyme is unable to synthesize consecutive α(1→6) glucosidic bonds. Instead it forms a high molecular mass α(1→4,6) branched reuteran like polymer with 68 % α(1→4), and 32 % α(1→6) glycosidic linkages using amylose V as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of glucosee from amylose. One unit of enzyme activity is defined as the release of 1 μmol of glucose per minute.
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    Azotobacter chroococcum

    n.a.

    2.4.1.-

    GH70

    6,5

    60

    Solid lyophilized powder

    86

    4

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  • The GtfC enzyme of Exiguobacterium sibiricum 255-15 is member of the GH70 family. This glucanotransferase is active on malto-oligosaccharides and starch, synthesizing isomalto/malto-oligosaccharides (IMMOs) containing 52 % α(1→6) and  35 % α(1→4)-glycosidic linkages using amylose V.

    This product is sold for research use only.
    *Activity was measured by following the release of glucose from amylose. One unit of enzyme activity is defined as the release of 1 μmol of glucose per minute.
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    Exiguobacterium sibiricum strain 255-15

    n.a.

    2.4.1.-

    GH70

    5,0

    40

    Solid lyophilized powder

    99

    4

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  • The C-terminal truncated levansucrase (Lev∆773) of Lactobacillus reuteri strain 121 belongs to the glycoside hydrolase family 68. This enzyme synthesizes a linear levan polymer containing β(2→6)- linked fructan units, and a low amount (2-3 %) of branching β(1→2→6) points, using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of glucose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of glucose per minute.
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    Lactobacillus reuteri strain 121

    Sucrose

    EC 2.4.1.10

    GH68

    5.4

    45

    Solid lyophilized powder

    88

    Room temperature

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  • The C-terminally truncated inulosucrase (Inu∆699) of Lactobacillus reuteri strain 121 is an fructosyltransferase belonging to the glycoside hydrolase family 68. This enzyme synthesizes a high molecular weight β(2→1)- linked fructan polymer of > 10 MDa, and oligosaccharides (95 % 1-kestose and 5 % nystose) using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of glucose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of glucose per minute.
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    Lactobacillus reuteri strain 121

    Sucrose

    EC 2.4.1.9

    GH68

    5.4

    55

    Solid lyophilized powder

    87

    Room temperature

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  • The N-terminally truncated glucansucrase GtfML1 of Lactobacillus reuteri strain ML1, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes a highly branched mutan-like polymer containing 65 % α(1→3), and 35 % α(1→6) linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri strain ML1

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    116

    4

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  • The N-terminally truncated glucansucrase Gtf33 of Lactobacillus parabuchneri 33, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with 81 % α(1→6) and 19 % α(1→3) linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
     
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    Lactobacillus parabuchneri strain 33

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    142

    4

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  • The N- terminally truncated glucansucrase GtfKg3 of Lactobacillus fermentum, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with 90 % α(1→6) and 10 % α(1→3) linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus fermentum strain Kg3

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    131

    4

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  • The N-terminally truncated glucansucrase GtfKg15 of Lactobacillus sakei, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with 90 % α(1→6), and 10 % α(1→3) linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus sakei strain Kg15

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    133

    4

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  • The N-terminally truncated enzyme of Lactobacillus reuteri strain ATCC 55730 (LB BIO) is a hydrolytic reuteransucrase producing a soluble glucan, in which the majority ~79 % of the linkages are of the α(1→4) glucosidic type . This reuteran also contains 21 % α(1→6)- linked glucosyl units, and 4,6-disubstituted α-glucosyl units at the branching points.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.

    Lactobacillus reuteri strain ATCC 55730

    Sucrose

    2.4.1-

    GH70

    5.0

    35

    Solid lyophilized powder

    117

    Room temperature

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  • The N- and V-terminally truncated glucansucrase Gtf180 of Lactobacillus reuteri 180, belongs to the glycosyl hydrolase family GH70. Compared to Gtf180-ΔN, Gtf180-ΔN-ΔV is impaired in polysaccharide synthesis. Instead this enzyme produces a large number of oligosaccharides. The enzyme forms α(1→6)- and α(1→3)-glycosidic linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri strain 180

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    50

    Solid lyophilized powder

    95

    4

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  • The N- terminally truncated glucansucrase GtfA of Lactobacillus reuteri strain 121, belongs to glycosyl hydrolase family GH70. The enzyme synthesizes branched glucans with 57 % α(1→4), and 43 % α(1→6)-glycosidic linkages (reuteran) using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri strain 121

    Sucrose

    2.4.1.-

    GH70

    4.7

    50

    Solid lyophilized powder

    118

    4

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  • The N-terminally truncated glucansucrase Gtf180 of Lactobacillus reuteri, belongs to glycosyl hydrolase family GH70. The enzyme synthesizes branched glucans containing 69 % α(1→6)-, and 31 % α(1→3)-glycosidic linkages (dextran) using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri strain 180

    Sucrose

    EC 2.4.1.5

    GH70

    4.5

    50

    Solid lyophilized powder

    117

    4

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  • The N-terminally- and domain V truncated enzyme GtfB of Lactobacillus reuteri strain 121 is member of the GH70 family. This 4,6-glucanotransferase is active on malto-oligosaccharides and starch, thereby yielding elongated isomalto-/malto-oligosaccharides (IMMO) and isomalto-/malto-polymers (IMMPs). The conversion of maltoheptaose (DP 7) using the GtfB-ΔN-ΔV ends up in linear products with a DP > 35,  containing 85 % α(1→4) and 15 % α(1→6) glycosidic linkages. The starch products formed by the GtfB enzyme are derived soluble dietary fibers, which are potentially useful in the food industry.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri strain 121

    Malto-oligosaccharides/ Amylose

    2.4.1.-

    GH70

    5,0

    40

    Solid lyophilized powder

    99

    4

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