The GtfML4 of Lactobacillus reuteri strain ML1 is a member of the glycoside hydrolase family GH70. The enzyme is a 4,6-α-glucanotransferase converting pure malto-oligosaccharide substrates (≥ DP 4) into linear α-glucan product mixtures with a glycosidic bonds ratio α(1→4): α(1→6) of 50%: 50% .

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*Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.