The Lactobacillus reuteri 180 N- and C-terminally truncated glucansucrase Gtf180, belongs to the glycosyl hydrolase family GH70. Compared to Gtf180-ΔN, Gtf180-ΔNΔV synthesizes a heavily impaired amount of polysaccharide containing α(1→3)- and α(1→6)-glycosidic linkages using sucrose as substrate. Instead this enzyme produces a large amount of oligosaccharides.

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*Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.