Carbohydrate-Active enZYmes

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  • Gtf180-ΔN dextransucrase

    175.00525.00

    The Lactobacillus reuteri 180 N-terminally truncated glucansucrase Gtf180, belongs to glycosyl hydrolase family GH70. The enzyme synthesizes branched glucans conaining α(1→3)- and α(1→6)-glycosidic linkages (dextran) using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri 180

    Sucrose

    EC 2.4.1.5

    GH70

    4.5

    50

    Solid lyophilized powder

    117

    4

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  • Gtf180-ΔN-ΔV dextransucrase

    175.00525.00

    The Lactobacillus reuteri 180 N- and C-terminally truncated glucansucrase Gtf180, belongs to the glycosyl hydrolase family GH70. Compared to Gtf180-ΔN, Gtf180-ΔNΔV synthesizes a heavily impaired amount of polysaccharide containing α(1→3)- and α(1→6)-glycosidic linkages using sucrose as substrate. Instead this enzyme produces a large amount of oligosaccharides.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri 180

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    50

    Solid lyophilized powder

    95

    4

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  • Gtf33-ΔN dextransucrase

    400.001,200.00

    The Lactobacillus parabuchneri 33 N-terminally truncated glucansucrase Gtf33, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with (1→6) linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
     
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    Lactobacillus parabuchneri 33

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    149

    4

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  • GtfA-ΔN reuteransucrase

    175.00525.00

    The Lactobacillus reuteri 121 N- terminally truncated glucansucrase GtfA, belongs to glycosyl hydrolase family GH70. The enzyme synthesizes branched glucans with α(1→4), and α(1→6)-glycosidic linkages (reuteran) using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri 121

    Sucrose

    2.4.1.-

    GH70

    4.7

    50

    Solid lyophilized powder

    118

    4

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  • GtfB-ΔN 4,6-α-glucanotransferase

    175.00525.00

    The N-terminally truncated enzyme GtfB of Lactobacillus reuteri strain 121 is member of the GH70 family. This 4,6-glucanotransferase is active on malto-oligosaccharides and starch, thereby yielding elongated isomalto/malto-oligomers (IMMOs)/polymers (IMMPs) containing α(1→4) and α(1→6) glycosidic linkages. The conversion of maltoheptaose (DP 7) using the GtfB of L. reuteri 121 ends up in products with a DP > 35.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri 121

    n.a.

    2.4.1.-

    GH70

    5,0

    40

    Solid lyophilized powder

    99

    4

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  • GtfC 4,6-α-glucanotransferase

    400.001,200.00

    The GtfC enzyme of Exiguobacterium sibiricum 255-15 is member of the GH70 family. This glucanotransferase is active on malto-oligosaccharides and starch, synthesizing isomalto/malto-oligosaccharides (IMMOs) containing α(1→4) and α(1→6)-glycosidic linkages.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Exiguobacterium sibiricum 255-15

    n.a.

    2.4.1.-

    GH70

    5,0

    40

    Solid lyophilized powder

    99

    4

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  • The GtfD enzyme of Azotobacter chroococcum is member of the glycoside hydrolase family GH70. The enzyme displays 4,6-α-glucanotransferase activity using malto-oligosaccharides (> DP 4), and starch. However, the enzyme is unable to synthesize consecutive  α(1→6) glucosidic bonds. Instead it forms a high molecular mass, α(1→4,6) branched reuteran like polymer.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Azotobacter chroococcum

    n.a.

    2.4.1.-

    GH70

    6,5

    60

    Solid lyophilized powder

    87

    4

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  • GtfKg15-ΔN dextransucrase

    400.001,200.00

    The Lactobacillus sakei N-terminally truncated glucansucrase GtfKg15, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with α(1→6) linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus sakei Kg15

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    133

    4

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  • GtfKg3-ΔN dextransucrase

    400.001,200.00

    The Lactobacillus fermentum Kg3 N- terminally truncated glucansucrase GtfKg3, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes mainly dextran-like polymers with (1→6) linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus fermentum Kg3

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    131

    4

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  • GtfML1-ΔN mutansucrase

    175.00525.00

    The Lactobacillus reuteri strain ML1 N-terminally truncated glucansucrase GtfML1, belongs to the glycosyl hydrolase family GH70. The enzyme synthesizes a highly branched mutan-like polymer containing α(1→3) (65 %) and α(1→6) (35 %) linkages using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri strain ML1

    Sucrose

    EC 2.4.1.5

    GH70

    5.5

    37

    Solid lyophilized powder

    116

    4

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  • gtfML4 4,6-α-glucanotransferase

    400.001,200.00

    The GtfML4 of Lactobacillus reuteri strain ML1 is a member of the glycoside hydrolase family GH70. The enzyme is a 4,6-α-glucanotransferase converting pure malto-oligosaccharide substrates (≥ DP 4) into linear α-glucan product mixtures with a glycosidic bonds ratio α(1→4): α(1→6) of 50%: 50% .

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri strain ML1

    Malto-oligosaccharides

    2.4.1.-

    GH70

    4,5

    40

    Solid lyophilized powder

    154

    4

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  • GtfO-ΔN reuteransucrase

    175.00525.00

    The enzyme of Lactobacillus reuteri strain ATCC 55730 (LB BIO) is a hydrolytic reuteransucrase producing a soluble glucan, in which the majority of the linkages are of the α-(1→4) glucosidic type (∼70%). This reuteran also contains α-(1→6)- linked glucosyl units and 4,6-disubstituted α-glucosyl units at the branching points.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.

    Lactobacillus reuteri ATCC 55730

    Sucrose

    2.4.1-

    GH70

    5.0

    35

    Solid lyophilized powder

    117

    Room temperature

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  • gtfW 4,6-α-glucanotransferase

    400.001,200.00

    The GtfW of Lactobacillus reuteri strain DSM 20016 is a member of the glycoside hydrolase GH70 family. The enzyme is a 4,6-α-glucanotransferase converting pure malto-oligosaccharide substrates into linear α-glucan product mixtures with a glycosidic bonds ratio of α(1→4): α(1→6) 50%: 50% .

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
    Select options

    Lactobacillus reuteri strain DSM 20016

    Malto-oligosaccharides

    2.4.1.-

    GH70

    4,5

    40

    Solid lyophilized powder

    180

    4

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  • The C-terminally truncated inulinosucrase (Inu∆699) of Lactobacillus reuteri strain 121 is an fructosyltransferase belonging to the glycoside hydrolase family 68. This enzyme synthesizes high molecular weight β(2→1)- linked fructan polymers of > 10 MDa, and oligosaccharides (95 % 1-kestose and 5 % nystose) using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
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    Lactobacillus reuteri 121

    Sucrose

    EC 2.4.1.9

    GH68

    5.0

    50

    Solid lyophilized powder

    87

    Room temperature

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  • The C-terminal truncated levansucrase (Lev∆773) of Lactobacillus reuteri strain 121 belongs to the glycoside hydrolase family 68. This enzyme synthesizes a linear levan polymer containing β(2→6)- linked fructan units, and a low amount (2-3 %) of branching β(1→2→6) points, using sucrose as substrate.

    This product is sold for research use only.
    *Activity was measured by following the release of fructose from sucrose. One unit of enzyme activity is defined as the release of 1 μmol of fructose per minute.
    Select options

    Lactobacillus reuteri 121

    Sucrose

    EC 2.4.1.10

    GH68

    5.0

    50

    Solid lyophilized powder

    88

    Room temperature

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