Publications 2019

J. Gangoiti, S.F. Corwin, L.M. Lamothe, C. Vafiadi, B.R. Hamaker, and L. Dijkhuizen (2020) – Synthesis of novel α-glucans with potential health benefits through controlled glucose release in the human gastrointestinal tract. Crit Rev Food Sci Nutr. 60: 123-146. doi: 10.1080/10408398.2018.1516621.

R.L. Valk-Weeber, L. Dijkhuizen, T. Eshuis-de Ruiter, S.S. van Leeuwen (2019) – Dynamic temporal variations in bovine lactoferrin glycan structures. J. Agric. Food Chem. doi: 10.1021/acs.jafc.9b06762.

M.C.L. Böger, S.S. van Leeuwen, A. Lammerts van Bueren and L. Dijkhuizen (2019) – Structural identity of the galactooligosaccharide molecules selectively utilized by single cultures of probiotic bacterial strains. J. Agr. Food Chem. 67: 13969-13977. doi: 10.1021/acs.jafc.9b05968.

R.L. Valk-Weeber, L. Dijkhuizen, S.S. van Leeuwen (2019) – Large-scale quantitative isolation of pure protein N-linked glycans. Carbohydr. Chem. 479: 13-22. doi: 10.1016/j.carres.2019.04.011.

T.T.H. Pham, G.A. ten Kate, L. Dijkhuizen, S.S. van Leeuwen (2019) – Synthesis and characterization of sialylated lactose- and lactulose-derived oligosaccharides by Trypanosoma cruzi trans-sialidase. J. Agr. Food Chem. 67: 3469-3479. doi: 10.1021/acs.jafc.8b06974.

M.C.L. Böger, J. Hekelaar, S.S. van Leeuwen, L. Dijkhuizen, and A. Lammerts van Bueren (2019) – Structural and functional characterization of a family GH53 β-1,4-galactanase from Bacteroides thetaiotaomicron that facilitates degradation of prebiotic galactooligosaccharides. J. Struct. Biol. 205: 1-10. doi: 10.1016/j.jsb.2018.12.002.

T.T.H. Pham, M.C.L. Boger, L. Dijkhuizen, and S.S. van Leeuwen (2019) – Stimulatory effects of novel glucosylated lactose derivatives GL34 on growth of selected gut bacteria. Appl. Microbiol. Biotechnol. 103: 707-718. doi: 10.1007/s00253-018-9473-8.

T. Devlamynck, E.M. te Poele, K. Quataert, G.J. Gerwig, D. van de Walle, K. Dewettinck, J.P. Kamerling, W. Soetaert, and L. Dijkhuizen (2019) – Trans-α-glucosylation of stevioside by the mutant glucansucrase enzyme Gtf180-ΔN-Q1140E improves its taste profile. Food Chem. 272: 653-662. doi: 10.1016/j.foodchem.2018.08.025.

Publications 2018

S. Figueroa-Lozano, R.L. Valk-Weeber, S.S. van Leeuwen, L. Dijkhuizen, P. de Vos (2018) – Dietary N-glycans from bovine lactoferrin and TLR modulation. Mol. Nutr. Food Res. 62. doi: 10.1002/mnfr.201700389.

J. Gangoiti, T. Pijning, and L. Dijkhuizen (2018) – Biotechnological potential of novel Glycoside Hydrolase family 70 enzymes synthesizing α-glucans from starch and sucrose. Biotechnol. Adv. 36:196-207. doi: 10.1016/j.biotechadv.2017.11.001

E.M. te Poele, T. Devlamynck, M. Jäger, G.J. Gerwig, D. van der Walle, K. Dewettinck, A.K.H. Hirsch, J.P. Kamerling, W. Soetaert, and L. Dijkhuizen (2018) – Glucansucrase (mutant) enzymes from Lactobacillus reuteri 180 efficiently transglucosylate Stevia component rebaudioside A, resulting in a superior taste. Sci. Rep. 8: 1516. doi: 10.1038/s41598-018-19622-5.

X. Meng, J. Gangoiti, N. de Kok, S.S. van Leeuwen, T. Pijning, and L. Dijkhuizen (2018) – Biochemical characterization of two GH70 family 4,6-α-glucanotransferases from Lactobacillus aviarius subsp. aviarius DSM 20655. Food Chemistry 253: 236-246. doi: 10.1016/j.foodchem.2018.01.154.

H. Yin, L. Dijkhuizen, and S.S. van Leeuwen (2018) – Synthesis of galacto-oligosaccharides derived from lactulose by wild-type and mutant β-galactosidase enzymes from Bacillus circulans ATCC 31382. Carbohydr. Res. 465: 58–65. https://doi.org/10.1016/j.carres.2018.06.009

X. Meng, J. Gangoiti, X. Wang, P. Grijpstra, S.S. van Leeuwen, T. Pijning, and L. Dijkhuizen (2018) – Biochemical characterization of a GH70 protein from Lactobacillus kunkeei DSM 12361 with two catalytic domains involving branching sucrase activity. Appl. Microbiol. Biotechnol. 102: 7935-7950.

T. Börner, J. Gangoiti, L. Dijkhuizen, S.S. van Leeuwen, L. Lamothe, C. Vafiadi, and S. Duboux (2018) – Discovery and development of novel glucanotransferases for healthier foods. In “Enzyme Engineering XXIV”, P. Monsan, Toulouse White Biotechnology, France; M. Remaud-Simeon, LISBP-INSA, University of Toulouse, France; Eds, ECI Symposium Series (2017).

E.M. te Poele, L. Dijkhuizen, G.J. Gerwig, J.P. Kamerling (2018) – Methods for the enzymatic modification of steviol glycosides, modified steviol glycosides obtainable thereby, and the use thereof as sweeteners. Patent Application WO2016144175A1.

M.C.L. Böger, L. Dijkhuizen, and A. Lammerts van Bueren (2018) – Cross-feeding among probiotic bacterial strains on prebiotic inulin involves the extracellular exo-inulinase of Lactobacillus paracasei strain W20. Appl. Environ. Microbiol. 84. pii: e01539-18. doi: 10.1128/AEM.01539-18.

T.T.H. Pham, L. Dijkhuizen, S.S. van Leeuwen (2018) – Structural characterization of glucosylated GOS derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes. Carbohydr. Res. 470:57-63. doi: 10.1016/j.carres.2018.10.003.

T.T.H. Pham, S.S. van Leeuwen, T. Pijning, and L. Dijkhuizen (2018) – Mutational analysis of the role of the glucansucrase Gtf180-ΔN active site residues in product and linkage specificity with lactose as acceptor substrate. J. Agr. Food Chem. 66: 12544-12554. doi: 10.1021/acs.jafc.8b04486.

S.S. van Leeuwen, E. Stoutjesdijk, G.A. ten Kate, A. Schaafsma, J. Dijck-Brouwer, F.A.J. Muskiet, and L. Dijkhuizen (2018) – Regional variations in human milk oligosaccharides in Vietnam suggest FucTx activity besides FucT2 and FucT3. Sci. Rep. 8:16790. doi: 10.1038/s41598-018-34882-x.

Publications 2017

X. Meng, T. Pijning, M. Tietema, J.M. Dobruchowska, H. Yin, G.J. Gerwig, S. Kralj, and L. Dijkhuizen (2017) – Characterization of the glucansucrase GTF180 W1065 mutant enzymes producing polysaccharides and oligosaccharides with altered linkage composition. Food Chem. 217: 81-90. doi: 10.1016/ j.foodchem.2016.08.087.

J. Gangoiti, S.S. van Leeuwen, S. Duboux, C. Vafiadi, G.J. Gerwig, T. Pijning, and L. Dijkhuizen (2017) – 4,3-α-Glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan GH-H. Nature Sci. Reports 7:39761. doi: 10.1038/ srep39761.

Y. Bai, J. Gangoiti, B.W. Dijkstra, L. Dijkhuizen, and T. Pijning (2017) – Crystal structure of 4,6-α-glucanotransferase supports diet-driven evolution of GH70 enzymes from α-amylases in oral bacteria. Structure 25: 231-242. doi: 10.1016/j.str.2016.11.023.

A. Lammerts van Bueren, M. Mulder, S. S. van Leeuwen and L. Dijkhuizen (2017) – Prebiotic galactooligosaccharides activate mucin and pectic galactan utilization pathways in the human gut symbiont Bacteroides thetaiotaomicron. Nature Sci. Reports 7:40478.

H. Yin, J. Bultema, L. Dijkhuizen, and S.S. van Leeuwen (2017) – Reaction kinetics and galactooligosaccharide product profiles of the β-galactosidases from Bacillus circulans, Kluyveromyces lactis and Aspergillus oryzae. Food Chem. 225: 230-238.

H. Yin, T. Pijning, X. Meng, L. Dijkhuizen, and S.S. van Leeuwen (2017) – Engineering of the Bacillus circulans β-galactosidase product specificity. ACS Biochemistry 56: 704-711.

V. Valk, R.M. van der Kaaij, and Lubbert Dijkhuizen (2017) – The evolutionary origin and possible functional roles of FNIII domains in two Microbacterium aurum B8.A granular starch degrading enzymes, and in other carbohydrate acting enzymes. Amylase 1: 1-11.

J. Gangoiti, X. Meng, A. Lammerts van Bueren, and L. Dijkhuizen (2017) – Draft genome sequence of Lactobacillus reuteri 121, a source of α-glucan and β-fructan exopolysaccharides. Genome Announc. 5(10), e01691-16. doi: 10.1128/genomeA.01691-16.

G.J. Gerwig, E.M. te Poele, L. Dijkhuizen, and J.P. Kamerling (2017) – Structural analysis of rebaudioside A derivatives obtained by Lactobacillus reuteri 180 glucansucrase-catalyzed trans-a-glucosylation. Carbohydr. Res.  440-441, 51-62.

E.M. te Poele, V. Valk, T. Devlamynck, S.S. van Leeuwen, and L. Dijkhuizen (2017) – Catechol glucosides act as donor/acceptor substrates of glucansucrase enzymes of Lactobacillus reuteri. Appl.Microbiol. Biotechnol. 101: 4495-4505. doi: 10.1007/s00253-017-8190-z.

J. Gangoiti, L. Lamothe, S.S. van Leeuwen , C. Vafiadi, and L. Dijkhuizen (2017) – Characterization of the Paenibacillus beijingensis DSM 24997 GtfD and its glucan polymer products representing a new Glycoside Hydrolase 70 subfamily of 4,6-α-glucanotransferase enzymes. PLOS One 12: e0172622.

F.D. Sarian, Š. Janeček, T. Pijning, Ihsanawati, Z. Nurachman, O.K. Radjasa , L. Dijkhuizen, D. Natalia and M.J.E.C. van der Maarel (2016) – The black sheep of the glycoside hydrolase family 13: a group of α-amylases with an aberrant catalytic triad. Sci. Rep. 7: 44230. doi: 10.1038/srep44230.

H. Yin, T. Pijning, X. Meng, L. Dijkhuizen, S.S. van Leeuwen (2017) – Biochemical characterization of the functional roles of residues in the active site of the β-galactosidase from Bacillus circulans ATCC 31382. Biochemistry 56: 3109-3118.

S. Montersino, E. te Poele, R. Orru, A.H. Westphal, A. Barendregt, A.J.R. Heck, R. van der Geize, L. Dijkhuizen, A. Mattevi, W.J.H. Van Berkel (2017) – 3-Hydroxybenzoate 6-hydroxylase from Rhodococcus jostii RHA1 contains a phosphatidylinositol cofactor. Front. Microbiol. 8: 1110. DOI: 10.3389/fmicb.2017.01110.

H.J. Frasch, S.S. van Leeuwen and L. Dijkhuizen (2017) – Molecular and biochemical characteristics of the inulosucrase HugO from Streptomyces viridochromogenes DSM 40736 (Tü494). Microbiology (SGM), 163: 1030-1041. DOI 10.1099/mic.0.000493.

H.T.T. Pham, L. Dijkhuizen and S.S van Leeuwen (2017) – Structural characterization of glucosylated lactose derivatives synthesized by the Lactobacillus reuteri GtfA and Gtf180 glucansucrase enzymes. Carbohydr. Res. 449: 59-64. DOI 10.1016/j.carres.2017.07.002.

A. Ceniceros, L. Dijkhuizen, M. Petrusma, and M.H. Medema (2017) – Genome-based exploration of the specialized metabolic capacities of the genus Rhodococcus. BMC Genomics 18: 593. DOI 10.1186/s12864-017-3966-1.

J. Gangoiti, S.S. van Leeuwen, S. Duboux, C. Vafiadi, T. Pijning, and L. Dijkhuizen (2017) – Mining novel starch-converting Glycoside Hydrolase 70 enzymes from the Nestlé Culture Collection genome database: The Lactobacillus reuteri NCC 2613 GtfB. Sci. Rep. 7: 9947. DOI: 10.1038/s41598-017-07190-z.

A. Ceniceros, L. Dijkhuizen and M. Petrusma (2017) – Molecular characterization of a Rhodococcus jostii RHA1 gamma-butyrolactone(-like) signalling molecule and its main biosynthesis gene gblA. Sci. Rep. 7: 17743. DOI: 10.1038/s41598-017-17853-6.

Publications 2016

X. Meng, J.M. Dobruchowska, T. Pijning, G.J. Gerwig, L. Dijkhuizen (2016) – Synthesis of new hyper-branched alpha-glucans from sucrose by Lactobacillus reuteri 180 glucansucrase mutants. J. Agr. Food Chem. 64: 433-442.

S.S. van Leeuwen, B. J. H. Kuipers, L. Dijkhuizen and J.P. Kamerling (2016) – Corrigendum to “1 H NMR analysis of the lactose/β-galactosidase derived galacto-oligosaccharide components of Vivinal® GOS up to DP5” [Carbohydr. Res. 400 (2014) 59–73]. Carbohydr. Res. 419: 69–70.

J. Gangoiti, S.S. van Leeuwen, C. Vafiadi, and L. Dijkhuizen (2016) – The Gram-negative bacterium Azotobacter chroococcum NCIMB 8003 employs a new Glycoside Hydrolase family 70 4,6-α-glucanotransferase enzyme (GTFD) to synthesize a reuteran like polymer from maltodextrins and starch. Biochim. Biophys. Acta 1860: 1224-1236.

E.M. te Poele, P. Grijpstra, S.S. van Leeuwen, and L. Dijkhuizen (2016) – Glucosylation of catechol with the GTFA glucansucrase enzyme from Lactobacillus reuteri and sucrose as donor substrate. Bioconjugate Chem. 27: 937-946.

S.S. van Leeuwen, B.J.H. Kuipers, L. Dijkhuizen, J.P. Kamerling (2016) – Comparative structural characterization of 7 commercial galacto-oligosaccharide (GOS) products. Carbohydr. Res. 425: 48-58.

Y. Bai, M.C.L. Böger, R.M. van der Kaaij, A.J.J. Woortman, T. Pijning, S.S. van Leeuwen, A. Lammerts van Bueren, and L. Dijkhuizen (2016) – Lactobacillus reuteri strains convert starch and maltodextrins into homo-exopolysaccharides using an extracellular and cell-associated 4,6-α-glucanotransferase. J. Agr. Food Chem. 64: 2941-2952.

T. Devlamynck, E.M. te Poele, X. Meng, S.S. van Leeuwen and L. Dijkhuizen (2016) – Glucansucrase Gtf180-ΔN of Lactobacillus reuteri 180: Enzyme and reaction engineering for improved glycosylation of non-carbohydrate molecules. Appl. Microbiol. Biotechnol. 100: 7529–7539.

V. Valk, A. Lammerts van Bueren, R.M. van der Kaaij, and L. Dijkhuizen (2016) – Carbohydrate Binding Module 74 is a novel starch-binding domain associated with large and multi-domain α-amylase enzymes. FEBS J. 2354-2368.

X. Meng, J. Gangoiti, Y. Bai, T. Pijning, S.S. van Leeuwen, and L. Dijkhuizen (2016) – Structure-function relationships of family GH70 glucansucrase and 4,6-α-glucanotransferase enzymes, and their evolutionary relationships with family GH13 enzymes. Cell. Mol. Life Sci. 73: 2681-2706.

Y. Bai, J.M. Dobruchowska, R.M. van der Kaaij, G.J. Gerwig,and L. Dijkhuizen (2016) – Structural basis for the roles of starch and sucrose in homo-exopolysaccharide formation by Lactobacillus reuteri 35-5. Carb. Polymers 151: 29-39.

J.M. Dobruchowska, J.O. Jonsson, O.H. Fridjonsson, A. Aevarsson, J.K. Kristjansson, J. Altenbuchner, H. Watzlawick, G.J. Gerwig, L. Dijkhuizen, J.P. Kamerling, and G.O. Hreggvidsson (2016) – Modification of linear (β1→3)-linked gluco-oligosaccharides with a novel recombinant β-glucosyltransferase (trans-β-glucosidase) enzyme from Bradyrhizobium diazoefficiens. Glycobiology 26(11):1157-1170. doi: 10.1093/ glycob/cww074.

G.J. Gerwig, E.M. te Poele, L. Dijkhuizen, and J.P. Kamerling (2016) – Stevia glycosides: Chemical and enzymatic modifications of their carbohydrate moieties to improve the sweet-tasting quality. Adv. Carbohydr. Chem. Biochem. 73: 1-72. doi: 10.1016/ bs.accb.2016.05.001.

X. Meng, T. Pijning, J.M. Dobruchowska1, H. Yin, G.J. Gerwig and L. Dijkhuizen (2016) – Structural determinants of alternating (α 1→4) and (α 1→6) linkage specificity in reuteransucrase of Lactobacillus reuteri. Sci. Reports 6: 35261. doi: 10.1038/ srep35261.

E.M. te Poele, L. Dijkhuizen, G. Gerwig, J.P. Kamerling (2016) – Methods for the enzymatic modification of steviol glycosides, modified steviol glycosides obtainable thereby, and the use thereof as sweeteners. Patent application WO 2016/144175 A1.

V. Valk, R.M. van der Kaaij, and Lubbert Dijkhuizen (2016) – Characterization of the starch-acting MaAmyB enzyme from Microbacterium aurum B8. A representing the novel subfamily GH13_42 with an enlarged catalytic region and an unusual and multidomain organization. Sci. Reports 6: 36100. doi: 10.1038/srep36100.

Y. Bai, J. Gangoiti, B.W. Dijkstra, L. Dijkhuizen, T. Pijning (2016) – Crystal structure of 4,6-α-glucanotransferase supports diet-driven evolution of GH70 enzymes from α-amylases in oral bacteria. Structure 25: 231–242. doi: 10.1016/j.str.2016.11.023.

Publications 2015

Y. Bai, R.M. van der Kaaij, A.J.J. Woortman, Z. Jin, L. Dijkhuizen (2015) – Characterization of the 4,6-α-glucanotransferase GTFB enzyme of Lactobacillus reuteri 121 isolated from inclusion bodies. BMC Biotechnol. 15: 49.

Y. Bai, R.M. van der Kaaij, H. Leemhuis, T. Pijning, S.S. van Leeuwen, Z. Jin, L. Dijkhuizen (2015) – Biochemical characterization of Lactobacillus reuteri Glycoside Hydrolase Family 70 GTFB type of 4,6-α-glucanotransferase enzymes that synthesize soluble dietary starch fibers. Appl. Envir. Microbiol. 81: 7223-7232.

J. Gangoiti, T. Pijning, and L. Dijkhuizen (2015) – Biochemical characterization of the Exiguobacterium sibiricum 255-15 GTFC enzyme representing a novel glycoside hydrolase 70 subfamily of 4,6-α-glucanotransferase enzymes. Appl. Environ. Microbiol. 82: 756-766.

Publications 2014

H. Leemhuis, J.M. Dobruchowska, M. Ebbelaar, F. Faber, P.L. Buwalda, M.J.E.C. van der Maarel , J.P. Kamerling, and L. Dijkhuizen (2014) – Isomalto/Malto Polysaccharide, a novel soluble dietary fiber made via enzymatic conversion of starch. J. Agric. Food Chem. 62: 12034-12044.

Publications 2013

H. Leemhuis, W.P. Dijkman, J.M. Dobruchowska, T. Pijning, P. Grijpstra, S. Kralj, J.P. Kamerling, L. Dijkhuizen (2013) – 4,6-a-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamily. Appl. Microbiol. Biotechnol. 97: 181-193.

Publications 2012

L. Dijkhuizen, M.J.E.C. van der Maarel, J.P. Kamerling, R.J. Leemhuis, S. Kralj and J.M. Dobruchowska (2012) – Glucooligosaccharides comprising (alpha1-4) and (alpha1-6) glycosidic bonds, use thereof, and methods for providing them. Patent application US 2012/0165290 A1.

J.M. Dobruchowska, G. Gerwig, H. Leemhuis, S. Kralj, P. Grijpstra, L. Dijkhuizen, J.P.
Kamerling (2012) – Structural characterization of the linear isomalto/malto oligomer products obtained by in vitro incubation of malto-oligomers with the recombinant GTFB glucanotransferase enzyme from Lactobacillus reuteri 121. Glycobiology 22: 517-528.

Publications 2011

S. Kralj, P. Grijpstra, S.S. van Leeuwen, H. Leemhuis, J.M. Dobruchowska, R.M. van der
Kaaij, A. Malik, A. Oetari, J.P. Kamerling and L. Dijkhuizen (2011) – 4,6-α-Glucanotransferase, a novel enzyme that structurally and functionally provides an evolutionary link between glycoside hydrolase enzyme families GH13 and GH70. Appl. Environ. Microbiol. 77: 8154-8163.

 

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